But implementations of this concept available thus far suffer from either high computational effort or costly results. To address these shortcomings, the concept of one-pass design for digital microfluidics biochips has recently been proposed-a holistic approach avoiding the design gaps by considering the whole synthesis process as large. Performing these steps consecutively may lead to design gaps and infeasible results. However, the design of such biochips still constitutes a challenging task that is usually tackled by multiple individual design steps, such as binding, scheduling, placement, and routing. Digital microfluidics biochips are a promising emerging technology that provides fluidic experimental capabilities on a chip (i.e., following the
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